Chhillar S., Snigh V.K., Kumar R., Atreja S.K.Microscopic and oxidative stress parameters after freeze-thawing process. (2007): The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen. Bucak M.N., Atessahin A., Varisli O., Yuce A., Tekin N., Akcay A.(2007): Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen. Journal of Microbiology, Biotechnology and Food Sciences, 2, 2168-2179. (2013): Semen quality assessment of New Zealand white rabbit bucks. Blaszczyk M., Slanina T., Massanyi P., Stawarz R.(1983): Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility. Keywords: amino acid liquid storage in vitro CASA system membrane integrity fowl The results indicated that the addition of taurine (from 2.5 to 7.5 mg/ml) to turkey spermatozoa positively affected the monitored spermatozoa parameters incubated at 5☌. A higher percentage of dead spermatozoa were observed at 41☌ (4.87-9.90%) if compared to 5☌ (2.12-4.88%). The analysed concentrations of taurine did not significantly affect viability of turkey spermatozoa during all time periods. Significantly lower values of motility parameters were detected in all experimental samples in comparison to the control after 5 h. The tendency of the taurine effect on motility parameters was different during the in vitro incubation at 41☌. After 2 h of incubation a significant preventive effect of taurine on spermatozoa parameters was observed. At 5☌, a significantly lower percentage of motility and progressive motility was detected only in the samples with the highest concentration of taurine (10 mg/ml) at time 0 and 1. Selected parameters were evaluated at six time periods: 0, 1, 2, 3, 4, and 5 h at 5☌ and 41☌. The motility parameters were evaluated by the CASA system (Computer Assisted Semen Analyser) using the program Sperm Vision ® and for spermatozoa viability assessment the eosin-nigrosin staining was performed. Experimental samples were prepared by diluting the raw semen in nine different concentrations of taurine - from 10 mg/ml to 0.078125 mg/ml. The effect of taurine on the turkey spermatozoa motility and viability during the in vitro incubation was assessed. Sci., 2018, 63(4):127-135 | DOI: 10.172-CJAS Effect of taurine on turkey ( Meleagris gallopavo) spermatozoa viability and motility Original Paper Tomáš Slanina *,1, Michal Miškeje 2, Filip Tirpák 1, Martyna Błaszczyk 3, Robert Stawarz 3, Peter Massányi 1,3 1ĭepartment of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovakia 2ĪgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Nitra, Slovakia 3ĭepartment of Zoology of Vertebrates and Human Biology, Institute of Biology, Pedagogical University of Krakow, Krakow, Poland
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